- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-41663r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Transmembrane protein 132A,Tmem132a
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Transmembrane protein 132A, TMEM132A ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TMEM132A,sGRP78 binding protein,GBP; HSPA5BP1; GRP78-binding protein; HSPA5-binding protein 1; glucose-regulated protein, 78kDa; heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) binding protein 1; heat shock 70kDa protein 5 binding protein 1; transmembrane protein 132A
Search name
Rat TMEM132A ELIAS KIT ,Rat sGRP78 binding protein ELIAS KIT ,Rat GBP ELIAS KIT ,Rat HSPA5BP1 ELIAS KIT ,Rat GRP78-binding protein ELIAS KIT ,Rat HSPA5-binding protein 1 ELIAS KIT ,Rat glucose-regulated protein ELIAS KIT ,Rat 78kDa ELIAS KIT ,Rat heat shock 70kDa protein 5 ELIAS KIT ,Rat glucose-regulated protein 78kDa binding protein 1 ELIAS KIT ,Rat heat shock 70kDa protein 5 binding protein 1 ELIAS KIT ,Rat transmembrane protein 132A ELIAS KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Transmembrane protein 132A, TMEM132A concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TMEM132A, During the reaction, TMEM132A in the sample or standard competes with a fixed amount of biotin-labeled TMEM132A for sites on a pre-coated Monoclonal antibody specific to TMEM132A. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TMEM132A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒？ dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好，我是新手，你用过的ELISA试剂在哪买的，哪个公司的比较好啊？还有，测定大鼠脑组织里的cAMP，放免法和ELISA法各有什么优缺点啊？谢谢回复！！ songdianwei 我在 R&D公司买的，测
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
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