Rat Zona pellucida sperm-binding protein 3, ZP3 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat ZP3 ELISA KIT ,Rat zona pellucida glycoprotein ZP3 ELISA KIT ,Rat ZP3A ELISA KIT ,Rat ZP3B ELISA KIT ,Rat ZPC ELISA KIT ,Rat Zp-3 ELISA KIT ,Rat ZP3A/ZP3B ELISA KIT ,Rat sperm receptor ELISA KIT ,Rat zona pellucida glycoprotein 3A (sperm receptor) ELISA KIT ,Rat zona pellucida glycoprotein 3B ELISA KIT ,Rat zona pellucida protein C ELISA KIT ,Rat zona pellucida glycoprotein 3 (sperm receptor) ELISA KIT
Intended use
Rat ZP3 ELISA kit allows for the in vitro quantitative determination of Rat ZP3 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat ZP3 ELISA kit has been pre-coated with an antibody specific to ZP3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ZP3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ZP3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ZP3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.31-20ng/ml
Rat ZP3 ELISA kit Manual download
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