- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-38901r
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Potassium/sodium hyperpolarization-activated cyclic nucleot
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Potassium/sodium hyperpolarization- activated cyclic nucleotide- gated channel 2, Hcn2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HCN2,BCNG-2; BCNG2; HAC-1; brain cyclic nucleotide gated channel 2; brain cyclic nucleotide-gated channel 2; potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2; hyperpolarization activated cyclic nucleotide-gated potassium channel 2,Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2,Brain cyclic nucleotide-gated channel 2,Hyperpolarization-activated cation channel 1,HAC-1
Search name
Rat HCN2 ELISA KIT ,Rat BCNG-2 ELISA KIT ,Rat BCNG2 ELISA KIT ,Rat HAC-1 ELISA KIT ,Rat brain cyclic nucleotide gated channel 2 ELISA KIT ,Rat brain cyclic nucleotide-gated channel 2 ELISA KIT ,Rat potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 ELISA KIT ,Rat hyperpolarization activated cyclic nucleotide-gated potassium channel 2 ELISA KIT ,Rat Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 ELISA KIT ,Rat Brain cyclic nucleotide-gated channel 2 ELISA KIT ,Rat Hyperpolarization-activated cation channel 1 ELISA KIT ,Rat HAC-1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2,HCN2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HCN2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HCN2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HCN2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HCN2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验15226268 REMARK GeneRIF: HO-1 interacts with caveolin-1 (inhibitory) and localizes in part to specialized plasma membrane caveolae in rat pulmonary artery endothelial cells. REFERENCE 12 (bases 1 to 1600) AUTHORS Huang,X.L., Ling,Y.L., Ling,Y.Q., Zhou
Measurement of Second Messengers in Signal Transduction: cAMP and Inositol Phosphates
. and Fillion, G. 1988. Reversed‐phase liquid chromatographic determination of cyclic adenosine 3′,5′‐monophosphate in rat brain cortex. J. Chromatogr. 426:177‐182. Gilman, A.G. 1970
Development of techniques for primary culture of C. elegans embryonic neurons
microscope or a Zeiss Axiophot microscope. For antibody staining, cultures were fixed for 30 min at room temperature in 4% paraformaldehyde in PBS, followed by three washes in PBS pH 7.2 containing 1% Triton X-100 and 1% BSA. Cultures
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