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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat CAPN2, m-calpain
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat CAPN2, m- calpain ELISA Kit
Product Name:Rat CAPN2, m- calpain ELISA Kit
Packing:96T
Catalog No.:ELA-E2249r
Gene Name:CAPN2, m-calpain
Detect Range:0.312-20 ng/mL
Sensitivity:0.1ng/mL
Target Protein Name:CAPN2, m-calpain
Alternative Name:CAPN2, m-calpain
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Rat CAPN2, m- calpain ELISA Kit allows for the in vitro quantitative determination of CAPN2, m-calpain concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验developed for in vitro studies (4 ). Recently, we have refined this approach to apply to in vivo studies of acute CNS injury (5 ,6 ). This technique allows for differential and concurrent measurement of the two major isoforms of calpain, m-calpain and μ
Rat Renal Proximal Tubules, Hypoxia, lonomycin, and Calpain
The preparation of freshly isolated rat renal proximal tubules in suspension has been utilized by our laboratory for more than 10 yr and reported in numerous publications (1 –10 ). This method of tubule isolation
人载脂蛋白M (APO M)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 APO M 单抗包被于酶标板上,标准品和样品中的 APO M与单抗结合,加入生物素化的抗人 APO M ,形成免疫复合物连接在板上,辣根
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