- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-28180r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Dihydrolipoyl dehydrogenase mitochondrial,Dld
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Dihydrolipoyl dehydrogenase mitochondrial, Dld ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Dihydrolipoyl dehydrogenase mitochondrial,Dihydrolipoamide dehydrogenase, Dld,GCSL,LAD,PHE3,Dihydrolipoamide dehydrogenase,Glycine cleavage system L protein,DLDD,DLDH,GCSL,LAD,PHE3,E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex,diaphorase,dihydrolipoyl dehydrogenase, mitochondrial,glycine cleavage system L protein,glycine cleavage system protein L,lipoamide dehydrogenase,lipoamide reductase,lipoyl dehydrogenase
Search name
Rat Dihydrolipoyl dehydrogenase mitochondrial ELISA KIT ,Rat Dihydrolipoamide dehydrogenase ELISA KIT ,Rat Dld ELISA KIT ,Rat GCSL ELISA KIT ,Rat LAD ELISA KIT ,Rat PHE3 ELISA KIT ,Rat Dihydrolipoamide dehydrogenase ELISA KIT ,Rat Glycine cleavage system L protein ELISA KIT ,Rat DLDD ELISA KIT ,Rat DLDH ELISA KIT ,Rat GCSL ELISA KIT ,Rat LAD ELISA KIT ,Rat PHE3 ELISA KIT ,Rat E3 component of pyruvate dehydrogenase complex ELISA KIT ,Rat 2-oxo-glutarate complex ELISA KIT ,Rat branched chain keto acid dehydrogenase complex ELISA KIT ,Rat diaphorase ELISA KIT ,Rat dihydrolipoyl dehydrogenase ELISA KIT ,Rat mitochondrial ELISA KIT ,Rat glycine cleavage system L protein ELISA KIT ,Rat glycine cleavage system protein L ELISA KIT ,Rat lipoamide dehydrogenase ELISA KIT ,Rat lipoamide reductase ELISA KIT ,Rat lipoyl dehydrogenase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat dihydrolipoyl dehydrogenase, mitochondrial,DLDH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to DLDH, During the reaction, DLDH in the sample or standard competes with a fixed amount of biotin-labeled DLDH for sites on a pre-coated Monoclonal antibody specific to DLDH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DLDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondrial dehydrogenase enzyme from viable cells to cleave the tetrazolium rings of the pale yellow MTT
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first described by Mosmann in 1983, is based on the ability of a mitochondrial dehydrogenase enzyme from viable cells to cleave the tetrazolium rings of the pale yellow MTT
线粒体荧光探针大全：TMRM，Mitotracker，JC-1
against the human pyruvate dehydrogenase (PDH) complex (Table 12.6), a large, multienzyme assembly residing in the mitochondrial matrix and consisting of three catalytic activities: pyruvate dehydrogenase, dihydrolipoyl transacetylase and dihydrolipoyl dehydrogenase
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