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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Adapter molecule crk,Crk
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Adapter molecule crk, CRK ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat CRK ELISA KIT ,Rat v-crk avian sarcoma virus CT10 oncogene homolog ELISA KIT ,Rat v-crk sarcoma virus CT10 oncogene-like protein ELISA KIT ,Rat proto-oncogene c-Crk ELISA KIT ,Rat CRKII ELISA KIT ,Rat p38 ELISA KIT ,Rat Adapter molecule crk ELISA KIT ,Rat CRKII ELISA KIT
Intended use
Rat CRK ELISA kit allows for the in vitro quantitative determination of Rat CRK concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat CRK ELISA kit has been pre-coated with an antibody specific to CRK. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CRK and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CRK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CRK in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Immunohistochemisty-Enzymatic Protocol
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Scintillation Proximity Assay (SPA) Technology to Study Biomolecular Interactions
as to bind specific molecules. If a radioactive molecule is bound to the bead, it is brought into close enough proximity that it can stimulate the scintillant contained within to emit light. Otherwise, the unbound radioactivity is too distant, the energy
Characterization of CGRP Receptor Binding
) 0.1 M NaOH 12‐well flat‐bottom plates γ counter with appropriate adapter
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