- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-24948r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat CDC42 small effector protein 1,Cdc42se1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat CDC42 small effector protein 1, CDC42SE1 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC42SE1,SCIP1; SPEC1; 1300002M12Rik; CDC42 small effector protein 1; CDC42-binding protein SCIP1; signaling molecule SPEC1 beta; small effector of CDC42 protein 1; small protein effector 1 of Cdc42; CDC42 small effector 1
Search name
Rat CDC42SE1 ELISA KIT ,Rat SCIP1 ELISA KIT ,Rat SPEC1 ELISA KIT ,Rat 1300002M12Rik ELISA KIT ,Rat CDC42 small effector protein 1 ELISA KIT ,Rat CDC42-binding protein SCIP1 ELISA KIT ,Rat signaling molecule SPEC1 beta ELISA KIT ,Rat small effector of CDC42 protein 1 ELISA KIT ,Rat small protein effector 1 of Cdc42 ELISA KIT ,Rat CDC42 small effector 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat CDC42 small effector protein 1, CDC42SE1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CDC42 small effector protein 1, CDC42SE1, During the reaction, Rat CDC42 small effector protein 1, CDC42SE1 in the sample or standard competes with a fixed amount of biotin-labeled Rat CDC42 small effector protein 1, CDC42SE1 for sites on a pre-coated Monoclonal antibody specific to Rat CDC42 small effector protein 1, CDC42SE1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rat CDC42 small effector protein 1, CDC42SE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Identification and Functional Reconstitution of Effector Proteins for the GTPases Rac and CDC42HS
Rho-related GTPase proteins, Rat 1,2, Rho A, B, G, and CDC42Hs, constitute a distinct subfamily in the Ras super family of GTPases ( 1 ) During the last few years a large body of evidence has been accumulated that suggests that Rho
Rac and Rap GTPase Activation Assays
by the development of high-affinity probes for small GTPases. These probes are created by fusing a high-affinity GTPase-binding domain derived from a specific downstream effector protein to glutathione-S -transferase (GST). Such domains bind preferentially
should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
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