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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Hyaluronidase PH-20,Spam1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Hyaluronidase PH- 20, SPAM1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Spam1,PH20,HEL-S-96n; HYA1; HYAL1; HYAL3; HYAL5; PH-20; PH20; SPAG15; epididymis secretory sperm binding protein Li 96n; hyal-PH20; hyaluronidase PH-20; hyaluronoglucosaminidase PH-20; sperm surface protein PH-20; sperm adhesion molecule
Search name
Rat Spam1 ELISA KIT ,Rat PH20 ELISA KIT ,Rat HEL-S-96n ELISA KIT ,Rat HYA1 ELISA KIT ,Rat HYAL1 ELISA KIT ,Rat HYAL3 ELISA KIT ,Rat HYAL5 ELISA KIT ,Rat PH-20 ELISA KIT ,Rat PH20 ELISA KIT ,Rat SPAG15 ELISA KIT ,Rat epididymis secretory sperm binding protein Li 96n ELISA KIT ,Rat hyal-PH20 ELISA KIT ,Rat hyaluronidase PH-20 ELISA KIT ,Rat hyaluronoglucosaminidase PH-20 ELISA KIT ,Rat sperm surface protein PH-20 ELISA KIT ,Rat sperm adhesion molecule ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Hyaluronidase PH- 20, SPAM1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Hyaluronidase PH-20. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Hyaluronidase PH-20 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Hyaluronidase PH-20, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Hyaluronidase PH-20 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product
Delivery time 4-6month
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
一、ELISA试剂盒的组成 完整的ELISA试剂盒包含以下各组分: (1)已包被抗原或抗体的固相载体(免疫吸附剂); (2)酶标记的抗原或抗体(结合物); (3)酶的底物; (4) 阴性对照品和阳性对照品(定性测定中),参考标准品和控制血清(定量测定中); (5)结合物及标本的稀释液; (6)洗涤液,在板式ELISA中,常用的稀释液为含0.05%吐温20磷酸缓冲盐水; (7)酶反应终止
inhibitors (Sigma Cat. no. P-8340). Chill this solution using wet ice. 6. Homogenize at low speed for ~20 seconds. Make sure to keep cool and keep samples on wet ice. 7. Transfer samples into 1.7 ml microcentrifuge tubes. 8. Centrifuge at 14,000 x g
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