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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Transient receptor potential cation channel subfamily V memb
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Transient receptor potential cation channel subfamily V member 1, TRPV1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TRPV1,VR1; OTRPC1; capsaicin receptor; osm-9-like TRP channel 1; transient receptor potential cation channel subfamily V member 1; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; vanilloid receptor subtype 1; transient receptor potential cation channel subfamily V member 1, Vanilloid receptor 1,Capsaicin receptor
Search name
Rat TRPV1 ELISA KIT ,Rat VR1 ELISA KIT ,Rat OTRPC1 ELISA KIT ,Rat capsaicin receptor ELISA KIT ,Rat osm-9-like TRP channel 1 ELISA KIT ,Rat transient receptor potential cation channel subfamily V member 1 ELISA KIT ,Rat transient receptor potential vanilloid 1a ELISA KIT ,Rat transient receptor potential vanilloid 1b ELISA KIT ,Rat vanilloid receptor subtype 1 ELISA KIT ,Rat transient receptor potential cation channel subfamily V member 1 ELISA KIT ,Rat Vanilloid receptor 1 ELISA KIT ,Rat Capsaicin receptor ELISA KIT,Rat Vanilloid receptor type 1-like ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Transient receptor potential cation channel subfamily V member 1, TrpV1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to TrpV1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for TrpV1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain TrpV1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of TrpV1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验to be essential for many biological functions including fluid and enzyme secretion, immune regulation, hypertension, pulmonary function, neurosecretion, synaptic plasticity, and vascular diseases. Canonical transient receptor potential (TRPCs) have been proposed
TRPM2 Function and Potential as a Drug Target
The TRPM subfamily of transient receptor potential channels includes a number of members which are involved in cell proliferation or cell survival. TRPM2, the second member to be cloned, has a key role in the response to oxidative stress
Hypoxia-Dependent TRP Channel Function in Pulmonary Arterial Smooth Muscle Cells
-2 in isolated wild-type (WT) and gene-deficient mouse PASMC. We also describe here the manganese-quenching method to determine the role of nonselective cation influx through transient receptor potential (TRP) channels in response to hypoxia.
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