- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-32279r
- 2025年07月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Elongation factor 2,Eef2
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Elongation factor 2, EEF2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Elongation factor 2,EF-2, EEF2,eukaryotic translation elongation factor 2; elongation factor 2; EEF-2; EF-2; EF2; polypeptidyl-tRNA translocase
Search name
Rat Elongation factor 2 ELISA KIT ,Rat EF-2 ELISA KIT ,Rat EEF2 ELISA KIT ,Rat eukaryotic translation elongation factor 2 ELISA KIT ,Rat elongation factor 2 ELISA KIT ,Rat EEF-2 ELISA KIT ,Rat EF-2 ELISA KIT ,Rat EF2 ELISA KIT ,Rat polypeptidyl-tRNA translocase ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat elongation factor 2,EF-2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to EF-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for EF-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain EF-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of EF-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验/ionization time of flight mass spectrometry as protein disulfide isomerase, one as 60 kDa heat-shock protein, and two as elongation factor Tu.
cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒？ dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好，我是新手，你用过的ELISA试剂在哪买的，哪个公司的比较好啊？还有，测定大鼠脑组织里的cAMP，放免法和ELISA法各有什么优缺点啊？谢谢回复！！ songdianwei 我在 R&D公司买的，测
组织充分那就可以用这些试剂盒检测。 当然，不同公司cAMP试剂盒灵敏度有些差异，这个也需要做好选择。 另外，选择这些试剂盒时还需要根据自己实验室的仪器配置来选择，例如Cisbio,跟PE公司的cAMP试剂盒是基于TR-FRET方法的，因此酶标仪必须有TRF跟FRET功能；Promega公司的试剂盒是化学发光的，因此仪器上需要有Luminescence; DiscoverX的试剂盒也是测的Luminescence；其他一些公司就是Elisa试剂盒，这个大家就比较
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