- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-32269r
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Dual specificity protein phosphatase 1,Dusp1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Dual specificity protein phosphatase 1, DUSP1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Dual specificity protein phosphatase 1, Protein-tyrosine phosphatase CL100,Dual specificity protein phosphatase
hVH1,Mitogen-activated protein kinase phosphatase 1(MAP kinase phosphatase 1,MKP-1), MKP-1,CL100; HVH1; MKP1; PTPN10; MAP
kinase phosphatase 1; dual specificity protein phosphatase 1; dual specificity protein phosphatase hVH1; mitogen-activated protein
kinase phosphatase 1; protein-tyrosine phosphatase CL100; serine/threonine specific protein phosphatase; dual specificity phosphatase
1, CL100;MKP1;PTPN10;VH1
Search name
Rat Dual specificity protein phosphatase 1 ELISA Kit, Rat DUSP1 ELISA Kit, Rat Protein-tyrosine phosphatase CL100
ELISA Kit, Rat Dual specificity protein phosphatase hVH1 ELISA Kit, Rat Mitogen-activated protein kinase phosphatase 1
ELISA Kit, Rat MAP kinase phosphatase 1 ELISA Kit, Rat MKP-1 ELISA Kit, Rat CL100 ELISA Kit, Rat HVH1 ELISA
Kit, Rat MKP1 ELISA Kit, Rat PTPN10 ELISA Kit, Rat serine/threonine specific protein phosphatase ELISA Kit, Rat
PTPN10 ELISA Kit, Rat VH1 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat dual specificity protein phosphatase
1,DUSP1concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to DUSP1. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DUSP1and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain DUSP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DUSP1in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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文献和实验Functional Studies of Dual-Specificity Phosphatases
Dual-speclflcity phosphatases, a subfamily enzymes of protein tyrosine phosphatases, play important roles in signal transduction and cell-cycle regulation during cell growth and differentiation (1 ). Unlike classic tyrosme phosphatases
Discovery of Protein Kinase Phosphatase Inhibitors
with chemical biology and high-content multiparametric analyses and was developed as a screening approach to discover protein phosphatase inhibitors. Using a dual specificity mitogen-activated protein kinase phosphatase as an example, a detailed protocol
Dual- and Triple-Color Fluorospot
实验原理 ELISPOT has, due to its high sensitivity and simplicity, proven valuable for assessing antigen-specific T-cell responses, both with regard to specificity and magnitude. One limitation of the regular ELISPOT method
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