- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-30900r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Histone deacetylase 7,Hdac7
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Histone deacetylase 7, HDAC7 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
HDAC7,HD7A; HDAC7A; HD7; histone deacetylase 7A; histone deacetylase 7
Search name
Rat HDAC7 ELISA KIT ,Rat HD7A ELISA KIT ,Rat HDAC7A ELISA KIT ,Rat HD7 ELISA KIT ,Rat histone deacetylase 7A ELISA KIT ,Rat histone deacetylase 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Histone deacetylase 7, HDAC7 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Histone deacetylase 7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Histone deacetylase 7 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone deacetylase 7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Histone deacetylase 7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验are explained here for rat liver. Critical parameters for the successful isolation of primary rat hepatocytes are highlighted and a troubleshooting guide is provided. In addition, a new development based on the inhibition of histone deacetylase activity
HDAC（Histone Deacetylase）组蛋白去乙酰化酶活性测定及药物筛选方案
DAC（Histone Deacetylase）组蛋白去乙酰化酶活性测定及药物筛选方组蛋白去乙酰化酶 （histone deacetylase,HDAC ）是一类蛋白酶,对染色体的结构修饰和基因表达调控发挥着重要的作用。一般情况下,组蛋白的乙酰化有利于DNA与组蛋白八聚体的解离，核小体结构松弛，从而使各种转录因子和协同转录因子能与DNA结合位点特异性结合，激活基因的转录。在细胞核内，组蛋白乙酰化与组蛋白去乙酰化过程处于动态平衡，并由组蛋白乙酰化转移 酶（histone
浅析染色质免疫沉淀（ChIP）技术在 DNA 与蛋白质相互作用研究中的重要性
, Y., et al., GATA and Nkx factors synergistically regulate tissue-specific gene expression and development in vivo. Development,2007. 134(1): p. 189-98. [2] Rossi, V., et al., A maize histone deacetylase and retinoblastoma-related protein physically
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