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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Polypyrimidine tract-binding protein 1,Ptbp1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Polypyrimidine tract- binding protein 1, Ptbp1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
PTBP1, PYBP,HNRNP-I; HNRNPI; HNRPI; PTB; PTB-1; PTB-T; PTB2; PTB3; PTB4; pPTB; Polypyrimidine tract-binding protein 1,57 kDa RNA-binding protein PPTB-1; RNA-binding protein; heterogeneous nuclear ribonucleoprotein I; heterogeneous nuclear ribonucleoprotein polypeptide I; hnRNP I; polypyrimidine tract binding protein,heterogeneous nuclear ribonucleoprotein I; polypyrimidine tract binding protein 1
Search name
Rat PTBP1 ELISA KIT ,Rat PYBP ELISA KIT ,Rat HNRNP-I ELISA KIT ,Rat HNRNPI ELISA KIT ,Rat HNRPI ELISA KIT ,Rat PTB ELISA KIT ,Rat PTB-1 ELISA KIT ,Rat PTB-T ELISA KIT ,Rat PTB2 ELISA KIT ,Rat PTB3 ELISA KIT ,Rat PTB4 ELISA KIT ,Rat pPTB ELISA KIT ,Rat Polypyrimidine tract-binding protein 1 ELISA KIT ,Rat 57 kDa RNA-binding protein PPTB-1 ELISA KIT ,Rat RNA-binding protein ELISA KIT ,Rat heterogeneous nuclear ribonucleoprotein I ELISA KIT ,Rat heterogeneous nuclear ribonucleoprotein polypeptide I ELISA KIT ,Rat hnRNP I ELISA KIT ,Rat polypyrimidine tract binding protein ELISA KIT ,Rat heterogeneous nuclear ribonucleoprotein I ELISA KIT ,Rat polypyrimidine tract binding protein 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination ofratPTBconcentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTB. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PTB and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PTB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PTB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验purification. The intronic splicing silencer (ISS) element present upstream of the N1 exon of the c-src pre-mRNA is a high-affinity binding site for the polypyrimidine tract binding protein (PTB). Using a 5′-biotinylated ISS RNA and PTB as an example
Detecting Low‐Affinity Extracellular Protein Interactions Using Protein Microarrays
extracellular protein interactions are critical for cellular recognition processes, but are not generally detected by methods that can be applied in a high?throughput manner. This unit describes a protein microarray platform that significantly improves
The Perinucleolar Compartment (PNC): Detection by Immunohistochemistry
by a immunohistochemical technique using the mouse monoclonal antibody SH54 (3) , which specifically recognizes the RNA binding protein PTB (polypyrimidine tract binding protein), which is highly concentrated in the PNC and is used as a marker for PNC detection.
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