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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat 25-hydroxycholesterol 7-alpha-hydroxylase,Cyp7b1 ELISA K
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat 25-hydroxycholesterol 7-alpha-hydroxylase, Cyp7b1 ELISA Kit
FOR RESEARCH USE ONLY!
96 Tests
Alternative name
Rat Cyp7b1 ELISA KIT ,Rat CBAS3 ELISA KIT ,Rat CP7B ELISA KIT ,Rat SPG5A ELISA KIT ,Rat cytochrome P450 7B1 ELISA KIT ,Rat cytochrome P450 family 7 subfamily B polypeptide 1 ELISA KIT ,Rat cytochrome P450 subfamily VIIB (oxysterol 7 alpha-hydroxylase) polypeptide 1 ELISA KIT ,Rat oxysterol 7-alpha-hydroxylase ELISA KIT ,Rat cytochrome P450 family 7 subfamily B member 1 ELISA KIT ,Rat 25-hydroxycholesterol 7-alpha-hydroxylase ELISA KIT ,Rat Oxysterol 7-alpha-hydroxylase ELISA KIT ,Rat Oxysterol 7-alpha-hydroxylase ELISA KIT ,Rat Cytochrome P450 7B1 ELISA KIT ,Rat Hippocampal transcript 1 protein ELISA KIT ,Rat HCT-1 ELISA KIT
Intended use
Rat Cyp7b1 ELISA kit allows for the in vitro quantitative determination of Rat Cyp7b1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat Cyp7b1 ELISA kit has been pre-coated with an antibody specific to Cyp7b1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyp7b1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyp7b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyp7b1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 78-5000pg/ml
Rat Cyp7b1 ELISA kit Manual download
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