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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Segment polarity protein dishevelled homolog DVL-1,Dvl1 EL
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Segment polarity protein dishevelled homolog DVL- 1, DVL1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
DVL1锛孌VL; DVL1L1; DVL1P1; DSH homolog 1; dishevelled 1 (homologous to Drosophila dsh); dishevelled, dsh homolog 1; dishevelled-1; segment polarity protein dishevelled homolog DVL-1; dishevelled segment polarity protein 1,Segment polarity protein dishevelled homolog DVL-1,Dishevelled-1,DSH homolog 1,
Search name
Rat DVL1 ELISA KIT ,Rat DVL ELISA KIT ,Rat DVL1L1 ELISA KIT ,Rat DVL1P1 ELISA KIT ,Rat DSH homolog 1 ELISA KIT ,Rat dishevelled 1 (homologous to Drosophila dsh) ELISA KIT ,Rat dishevelled ELISA KIT ,Rat dsh homolog 1 ELISA KIT ,Rat dishevelled-1 ELISA KIT ,Rat segment polarity protein dishevelled homolog DVL-1 ELISA KIT ,Rat dishevelled segment polarity protein 1 ELISA KIT ,Rat Segment polarity protein dishevelled homolog DVL-1 ELISA KIT ,Rat Dishevelled-1 ELISA KIT ,Rat DSH homolog 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat segment polarity protein dishevelled homolog DVL-1, Dishevelled-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dishevelled-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dishevelled-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dishevelled-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dishevelled-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验v468.chapter 1 经典 Wnt/b-Catenin 信号通路 (英文完整版)
., Wagenaar, E.,Parren, P., Weigel, D., and Nusse R. (1987) The Drosophila homolog of the mouse mammary oncogene int-1 is identical to the segment polarity gene wingless. Cell 50 , 649–657. 3. McMahon, A. P. and Moon, R. T. (1989) Ectopic expression
别再只测β‑catenin!完整搞懂Wnt经典与非经典信号通路
,FZD通常还需要与LRP5或LRP6共同参与信号传递。Wnt与FZD和LRP5/6形成受体复合物后,会进一步引起胞内信号变化。 在细胞内部,Wnt信号还涉及多种调控蛋白,例如Dishevelled(DVL)、AXIN、APC、CK1、GSK-3β和β-catenin等。其中部分蛋白主要参与经典Wnt/β-catenin通路,而其他信号分支则具有不同的下游分子。 因此,从最基本的层面来看,Wnt信号可以理解为:Wnt配体 → 细胞膜受体/共受体 → 胞内信号转导 → 基因表达或细胞行为改变
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