Rat Segment polarity protein d

Rat Segment polarity protein dishevelled homolog DVL- 1, DVL1 ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DVL1锛孌VL; DVL1L1; DVL1P1; DSH homolog 1; dishevelled 1 (homologous to Drosophila dsh); dishevelled, dsh homolog 1; dishevelled-1; segment polarity protein dishevelled homolog DVL-1; dishevelled segment polarity protein 1,Segment polarity protein dishevelled homolog DVL-1,Dishevelled-1,DSH homolog 1,

Search name

Rat DVL1 ELISA KIT ,Rat DVL ELISA KIT ,Rat DVL1L1 ELISA KIT ,Rat DVL1P1 ELISA KIT ,Rat DSH homolog 1 ELISA KIT ,Rat dishevelled 1 (homologous to Drosophila dsh) ELISA KIT ,Rat dishevelled ELISA KIT ,Rat  dsh homolog 1 ELISA KIT ,Rat dishevelled-1 ELISA KIT ,Rat segment polarity protein dishevelled homolog DVL-1 ELISA KIT ,Rat dishevelled segment polarity protein 1 ELISA KIT ,Rat Segment polarity protein dishevelled homolog DVL-1 ELISA KIT ,Rat Dishevelled-1 ELISA KIT ,Rat DSH homolog 1 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat segment polarity protein dishevelled homolog DVL-1, Dishevelled-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dishevelled-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Dishevelled-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Dishevelled-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Dishevelled-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.