Rat Cytochrome c testis- speci

Rat Cytochrome c testis- specific, Cyct ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Cytochrome c 2,Cyct,cytochrome c, testis-specific; T-Cc; cytochrome c, testis

Search name

Rat Cytochrome c 2 ELISA KIT ,Rat Cyct ELISA KIT ,Rat cytochrome c testis-specific ELISA KIT ,Rat T-Cc ELISA KIT ,Rat cytochrome c testis ELISA KIT ,

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Cytochrome c testis- specific, Cyct concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyct. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyct and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyct, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyct in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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