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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Runt-related transcription factor 2,Runx2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Runt-related transcription factor 2, RUNX2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Runt-related transcription factor 2,SL3-3 enhancer factor 1 alpha A subunit,Polyomavirus enhancer-binding protein 2 alpha A subunit,PEA2-alpha A,PEBP2-alpha A,Osteoblast-specific transcription factor 2,OSF-2,Oncogene AML-3,SL3/AKV core-binding factor alpha A subunit,Acute myeloid leukemia 3 protein,Core-binding factor subunit alpha-1,CBF-alpha-1,RUNX2,runt-related transcription factor 2; AML3; CBF-alpha-1; CBFA1; CCD; CCD1; CLCD; OSF-2; OSF2; PEA2aA; PEBP2aA; PEA2-alpha A; PEBP2-alpha A; SL3-3 enhancer factor 1 alpha A subunit; SL3/AKV core-binding factor alpha A subunit; acute myeloid leukemia 3 protein; core-binding factor, runt domain, alpha subunit 1; oncogene AML-3; osteoblast-specific transcription factor 2; polyomavirus enhancer-binding protein 2 alpha A subunit
Search name
Rat Runt-related transcription factor 2 ELISA KIT ,Rat SL3-3 enhancer factor 1 alpha A subunit ELISA KIT ,Rat Polyomavirus enhancer-binding protein 2 alpha A subunit ELISA KIT ,Rat PEA2-alpha A ELISA KIT ,Rat PEBP2-alpha A ELISA KIT ,Rat Osteoblast-specific transcription factor 2 ELISA KIT ,Rat OSF-2 ELISA KIT ,Rat Oncogene AML-3 ELISA KIT ,Rat SL3/AKV core-binding factor alpha A subunit ELISA KIT ,Rat Acute myeloid leukemia 3 protein ELISA KIT ,Rat Core-binding factor subunit alpha-1 ELISA KIT ,Rat CBF-alpha-1 ELISA KIT ,Rat RUNX2 ELISA KIT ,Rat runt-related transcription factor 2 ELISA KIT ,Rat AML3 ELISA KIT ,Rat CBF-alpha-1 ELISA KIT ,Rat CBFA1 ELISA KIT ,Rat CCD ELISA KIT ,Rat CCD1 ELISA KIT ,Rat CLCD ELISA KIT ,Rat OSF-2 ELISA KIT ,Rat OSF2 ELISA KIT ,Rat PEA2aA ELISA KIT ,Rat PEBP2aA ELISA KIT ,Rat PEA2-alpha A ELISA KIT ,Rat PEBP2-alpha A ELISA KIT ,Rat SL3-3 enhancer factor 1 alpha A subunit ELISA KIT ,Rat SL3/AKV core-binding factor alpha A subunit ELISA KIT ,Rat acute myeloid leukemia 3 protein ELISA KIT ,Rat core-binding factor ELISA KIT ,Rat runt domain ELISA KIT ,Rat alpha subunit 1 ELISA KIT ,Rat oncogene AML-3 ELISA KIT ,Rat osteoblast-specific transcription factor 2 ELISA KIT ,Rat polyomavirus enhancer-binding protein 2 alpha A subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat runt-related transcription factor 2,Runt-related transcription factor 2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Runt-related transcription factor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Runt-related transcription factor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Runt-related transcription factor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Runt-related transcription factor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验, as the disappearance of complexes is observed only with the addition of WT but not MT competitors. RUNX proteins are the major component of the transcription factor complex binding to Gallus BSP promoter (B) . Electrophoretic mobility antibody supershift analyses
mechanisms of its transcription, we have cloned the rat α1B -AR gene and identified its promoters and the cis -acting elements in its 5′ regulatory domain (2 ). Footprinting analyses using liver nuclear extracts have revealed a strongly protected region
浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
。结果显示 GSTP1 基因在处理组和非处理组之间有显著差异,这表明 Se 处理的癌细胞 GSTP1 启动子相关的 H3-K9 乙酰化水平提高、 H3-K3 甲基化水平降低,预示 Se 可能具有调节染色质表观结构的功能。 正因为 ChIP 技术对于表观遗传学及信号转导研究是如此之重要,默克密理博特别为该领域的科学家们专门开发了的 ChIP 解决方案,其中完备的 ChIP 试剂盒免去提前准备各种试剂(包括鲑鱼精子 DNA 包被的琼脂糖珠子、Protein A/G 等)的麻烦,专用的 ChIP 抗体
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