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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat NADPH oxidase 1,Nox1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat NADPH oxidase 1,NOX1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
NOX1,NOX-1,MOX-1,GP91-2; MOX1; NOH-1; NOH1; NADH/NADPH mitogenic oxidase subunit P65-MOX; NADPH oxidase homolog-1; mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating); mitogenic oxidase 1; NADPH oxidase 1
Search name
Rat NOX1 ELISA KIT ,Rat NOX-1 ELISA KIT ,Rat MOX-1 ELISA KIT ,Rat GP91-2 ELISA KIT ,Rat MOX1 ELISA KIT ,Rat NOH-1 ELISA KIT ,Rat NOH1 ELISA KIT ,Rat NADH/NADPH mitogenic oxidase subunit P65-MOX ELISA KIT ,Rat NADPH oxidase homolog-1 ELISA KIT ,Rat mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating) ELISA KIT ,Rat mitogenic oxidase 1 ELISA KIT ,Rat NADPH oxidase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat nadph oxidase 1,NOX-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to NOX-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for NOX-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain NOX-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of NOX-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Assessment of the Role for Rho Family GTPases in NADPH Oxidase Activation
Rac, a member of the Rho family small GTPases, plays a crucial role in activation of Nox family NADPH oxidases in animals, enzymes dedicated to production of reactive oxygen species such as superoxide. The phagocyte oxidase Nox2, crucial
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
a. Endothelial NADPH oxidase as the source of oxidants in lungs exposed to ischemia or high K+. Circ. Res. 83:730‐737. Al‐Mehdi, A.B., Zhao, G., and Fisher, A.B. 1998b. ATP‐independent
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