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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Microtubule-associated proteins 1A/1B light chain 3B,Map1l
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Microtubule- associated proteins 1A/1B light chain 3B, MAP1LC3B ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY ELISA KIT, NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
MAP1LC3B ELISA KIT,MAP1LC3B-a ELISA KIT, ATG8F ELISA KIT, LC3B ELISA KIT, MAP1A/1BLC3 ELISA KIT, MAP1 light chain 3-like protein 2 ELISA KIT, MAP1A/MAP1B LC3 B ELISA KIT, MAP1A/MAP1B light chain 3 B ELISA KIT, autophagy-related ubiquitin-like modifier LC3 B ELISA KIT, microtubule-associated proteins 1A/1B light chain 3B ELISA KIT, microtubule-associated protein 1 light chain 3 beta.Autophagy-related protein LC3 B
Search name
Rat MAP1LC3B ELISA KIT ,Rat MAP1LC3B-a ELISA KIT ,Rat ATG8F ELISA KIT ,Rat LC3B ELISA KIT ,Rat MAP1A/1BLC3 ELISA KIT ,Rat MAP1 light chain 3-like protein 2 ELISA KIT ,Rat MAP1A/MAP1B LC3 B ELISA KIT ,Rat MAP1A/MAP1B light chain 3 B ELISA KIT ,Rat autophagy-related ubiquitin-like modifier LC3 B ELISA KIT ,Rat microtubule-associated proteins 1A/1B light chain 3B ELISA KIT ,Rat microtubule-associated protein 1 light chain 3 beta ELISA KIT ,Rat Autophagy-related protein LC3 B ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Microtubule-associated proteins 1A/1B light chain 3B concentrations in serum ELISA KIT, Plasma ELISA KIT, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule-associated proteins 1A/1B light chain 3B. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Microtubule-associated proteins 1A/1B light chain 3B and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Microtubule-associated proteins 1A/1B light chain 3B ELISA KIT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Microtubule-associated proteins 1A/1B light chain 3B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验微管结合蛋白(microtubule-associated proteins, MAPs)
与微管特异地结合在一起, 对微管的功能起辅助作用的蛋白质称为微管结合蛋白, 在微管结构中约占10~15%。 一类主要的MAPs家族叫作装配MAPs(assembly MAPs), 作用是将微管在胞质溶胶中进行交联。这些MAPs的结构中具有两个结构域, 一个是碱性的微管蛋白结合结构域, 另一个是酸性的外伸的结构域。 根据序列特点, 将MAPs分成两个主要的类型:Ⅰ型和Ⅱ型(还有其他类型)。MAP1A和MAP1B含有几个重复的氨基酸序列:Lys-Lys-Glu
Chamberlin, N.L., Du, B., de Lacalle, S., and Saper, C.B. 1998. Recombinant adeno‐associated virus vector: Use for transgene expression and anterograde tract tracing in the CNS. Brain Res. 793:169‐175.
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of ∼17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic
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