- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-07268r
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Semaphorin-3A,Sema3a
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Semaphorin- 3A, SEMA3A ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Semaphorin-3A,Semaphorin III(Sema III),
Search name
Rat Semaphorin-3A ELISA Kit, Rat SEMA3A ELISA Kit,Semaphorin-3A ELISA Kit,SEMA3A ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat semaphorin-3a concentrations in serum, Plasma, Urine,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Semaphorin-3A. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Semaphorin-3A and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Semaphorin-3A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Semaphorin-3A in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Sema3A(Sema3A)ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Sema3A 单抗包被于酶标板上，标准品和样品中的 Sema3A 与单抗结合，加入生物素化的抗大鼠 Sema3A ，形成免疫复合物连接在板上，辣根过氧化物酶标记的 Streptavidin 与生物素结合
Morpholino-Mediated Gene Knockdown in Mammalian Organ Culture
We examined the role of semaphorin3a in ureteric bud (UB) branching morphogenesis using mouse metanephric organ culture [Tufro et al. (Mech Dev 125:558–568, 2008)]. In vitro UB injection of Sema3a antisense morpholino resulted in increased
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