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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Appetite-regulating hormone,Ghrl
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Appetite-regulating hormone, GHRL ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Appetite-regulating hormone,Protein M46,Motilin-related peptide,Growth hormone secretagogue,Growth hormone-releasing peptide, GHRL,ghrelin,MTLRP; appetite-regulating hormone; ghrelin, growth hormone secretagogue receptor ligand; ghrelin/obestatin preprohormone; growth hormone secretagogue; growth hormone-releasing peptide; motilin-related peptide; prepro-appetite regulatory hormone; ghrelin/obestatin prepropeptide
Search name
Rat Appetite-regulating hormone ELISA KIT ,Rat Protein M46 ELISA KIT ,Rat Motilin-related peptide ELISA KIT ,Rat Growth hormone secretagogue ELISA KIT ,Rat Growth hormone-releasing peptide ELISA KIT ,Rat GHRL ELISA KIT ,Rat ghrelin ELISA KIT ,Rat MTLRP ELISA KIT ,Rat appetite-regulating hormone ELISA KIT ,Rat ghrelin ELISA KIT ,Rat growth hormone secretagogue receptor ligand ELISA KIT ,Rat ghrelin/obestatin preprohormone ELISA KIT ,Rat growth hormone secretagogue ELISA KIT ,Rat growth hormone-releasing peptide ELISA KIT ,Rat motilin-related peptide ELISA KIT ,Rat prepro-appetite regulatory hormone ELISA KIT ,Rat ghrelin ELISA KIT,Rat obestatin prepropeptide ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Appetite-regulating hormone concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Appetite-regulating hormone, During the reaction, Appetite-regulating hormone in the sample or standard competes with a fixed amount of biotin-labeled Appetite-regulating hormone for sites on a pre-coated Monoclonal antibody specific to Appetite-regulating hormone. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Appetite-regulating hormone in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
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