Rat Dolichyl- diphosphooligosa

Rat Dolichyl- diphosphooligosaccharide - protein glycosyltransferase 48 kDa subunit, DDOST ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

DDOST,AGER1; CDG1R; OKSWcl45; OST; OST48; WBP1; advanced glycation endproduct receptor 1; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; dolichyl-diphosphooligosaccharide-protein glycotransferase; oligosaccharyl transferase 48 kDa subunit; oligosaccharyltransferase 48 kDa subunit; oligosaccharyltransferase subunit 48; dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit, DDOST 48 kDa subunit

Search name

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Intended use

This immunoassay kit allows for the in vitro quantitative determination of rat dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kda subunit, DDOST 48 kDa subunit concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to DDOST 48 kDa subunit. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DDOST 48 kDa subunit and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain DDOST 48 kDa subunit, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of DDOST 48 kDa subunit in the samples is then determined by comparing the O.D. of the samples to the standard curve.