Rat Mitochondrial uncoupling p

Rat Mitochondrial uncoupling protein 2,UCP 2 ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

UCP2,uncoupling protein 2,BMIQ4; SLC25A8; UCPH; solute carrier family 25 member 8; uncoupling protein 2

Search name

Rat UCP2 ELISA KIT ,Rat uncoupling protein 2 ELISA KIT ,Rat BMIQ4 ELISA KIT ,Rat SLC25A8 ELISA KIT ,Rat UCPH ELISA KIT ,Rat solute carrier family 25 member 8 ELISA KIT ,Rat uncoupling protein 2 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat Uncoupling Protein 2, Mitochondrial,UCP-2. concentrations in serum, tissue homogenates and other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to UCP-2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for UCP-2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UCP-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of UCP-2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

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