- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-06058r
- 2025年07月06日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Cyclin-dependent kinase 1,Cdk1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Cyclin- dependent kinase 1, Cdk1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CDC2,cell cycle controller CDC2; CDC28A; P34CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase;CDK1,CDC2;CDC28A;CDKN1;P34CDC2,Cyclin-dependent kinase 1,CDK1, p34 protein kinase;Cell division control protein 2 homolog;Cyclin-dependent kinase inhibitor 1,p21,CDK-interacting protein 1,Melanoma differentiation-associated protein,
Search name
Rat CDC2 ELISA KIT ,Rat cell cycle controller CDC2 ELISA KIT ,Rat CDC28A ELISA KIT ,Rat P34CDC2 ELISA KIT ,Rat cell division control protein 2 homolog ELISA KIT ,Rat cell division cycle 2 ELISA KIT ,Rat G1 to S and G2 to M ELISA KIT ,Rat cell division protein kinase 1 ELISA KIT ,Rat p34 protein kinase;CDK1 ELISA KIT ,Rat CDC2;CDC28A;CDKN1;P34CDC2 ELISA KIT ,Rat Cyclin-dependent kinase 1 ELISA KIT ,Rat CDK1 ELISA KIT ,Rat p34 protein kinase;Cell division control protein 2 homolog;Cyclin-dependent kinase inhibitor 1 ELISA KIT ,Rat p21 ELISA KIT ,Rat CDK-interacting protein 1 ELISA KIT ,Rat Melanoma differentiation-associated protein ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat cyclin-dependent kinase inhibitor 1,Cyclin-dependent kinase inhibitor 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclin-dependent kinase inhibitor 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cyclin-dependent kinase inhibitor 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase inhibitor 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cyclin-dependent kinase inhibitor 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
Cyclin-dependent kinases (CDK) are serine/threonine kinases that regulate cell cycle control and progression (1 ,2 ). A CDK holoenzyme complex is active if associated with its cyclin partner and if the complex is phosphorylated at specific
Analysis of Cyclin-Dependent Kinase Activity
are therefore needed to test chemical candidates for such therapy. Here we present a protocol for analysis of kinase activity for cyclin-dependent kinases using general substrates that will detect the broad activity of cyclin-dependent kinases (Fig. 1 ).
The cyclin/cyclin-dependent kinase (Cdk) complexes and the Cdk inhibitors (CDKI) are crucial regulators of cell cycle progression in all eukaryotic cells. Using rat cardiac myocytes as a model system, this chapter provides a detailed account
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