Rat Chitinase- 3- like protein

Rat Chitinase- 3- like protein 1, CHI3L1 ELISA KIT

96 Tests

Operating instructions

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!

Synonyms

Chitinase-3-like protein 1,YKL-40,39 kDa synovial protein,Cartilage glycoprotein 39,CGP-39,GP-39,hCGP-39, CHI3L1,ASRT7; CGP-39; GP-39; GP39; HC-gp39; HCGP-3P; YKL-40; YKL40; YYL-40; hCGP-39; 39 kDa synovial protein; cartilage glycoprotein 39; chitinase-3-like protein 1; chitinase 3-like 1 cartilage glycoprotein-39

Search name

Rat Chitinase-3-like protein 1 ELISA KIT ,Rat YKL-40 ELISA KIT ,Rat 39 kDa synovial protein ELISA KIT ,Rat Cartilage glycoprotein 39 ELISA KIT ,Rat CGP-39 ELISA KIT ,Rat GP-39 ELISA KIT ,Rat hCGP-39 ELISA KIT ,Rat  CHI3L1 ELISA KIT ,Rat ASRT7 ELISA KIT ,Rat CGP-39 ELISA KIT ,Rat GP-39 ELISA KIT ,Rat GP39 ELISA KIT ,Rat HC-gp39 ELISA KIT ,Rat HCGP-3P ELISA KIT ,Rat YKL-40 ELISA KIT ,Rat YKL40 ELISA KIT ,Rat YYL-40 ELISA KIT ,Rat hCGP-39 ELISA KIT ,Rat 39 kDa synovial protein ELISA KIT ,Rat cartilage glycoprotein 39 ELISA KIT ,Rat chitinase-3-like protein 1 ELISA KIT ,Rat chitinase 3-like 1 cartilage glycoprotein-39 ELISA KIT

Intended use

This immunoassay kit allows for the in vitro quantitative determination of Rat chitinase-3-like protein 1,Chitinase-3-like protein 1 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.

Test principle

The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chitinase-3-like protein 1, During the reaction, Chitinase-3-like protein 1 in the sample or standard competes with a fixed amount of biotin-labeled Chitinase-3-like protein 1 for sites on a pre-coated Monoclonal antibody specific to Chitinase-3-like protein 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Chitinase-3-like protein 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.