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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Gamma-glutamyltranspeptidase 1,Ggt1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Gamma-glutamyltranspeptidase 1,GGT 1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Gamma-glutamyltranspeptidase 1,Gamma-glutamyltransferase 1, GGT 1,glutamyl transpeptidase,CD224; D22S672; D22S732; GGT; GTG; gamma-glutamyltranspeptidase 1; glutathione hydrolase 1; leukotriene-C4 hydrolase; gamma-glutamyltransferase 1
Search name
Rat Gamma-glutamyltranspeptidase 1 ELISA KIT ,Rat Gamma-glutamyltransferase 1 ELISA KIT ,Rat GGT 1 ELISA KIT ,Rat glutamyl transpeptidase ELISA KIT ,Rat CD224 ELISA KIT ,Rat D22S672 ELISA KIT ,Rat D22S732 ELISA KIT ,Rat GGT ELISA KIT ,Rat GTG ELISA KIT ,Rat gamma-glutamyltranspeptidase 1 ELISA KIT ,Rat glutathione hydrolase 1 ELISA KIT ,Rat leukotriene-C4 hydrolase ELISA KIT ,Rat gamma-glutamyltransferase 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat gamma-glutamyltranspeptidase 1,GGT 1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GGT 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GGT 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GGT 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GGT 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验,表现出Th1、Th2和TFH等具有传统功能的细胞亚群。导致Foxp3下调的关键因素包括高水平的炎症因子内环境,如可诱导效应性T细胞产生的细胞因子:IL-6和IFN-gamma。Tregs 细胞中Foxp3的表达分析CD4+CD25+ Treg 细胞中Foxp3 +细胞群表明与Foxp3+Treg完全重合。使用Foxp3鉴定Treg用Anti-Mouse CD4 FITC、Anti-Mouse CD25 APC和Anti-Mouse/Rat Foxp3 PE 标记BALB/c小鼠 脾细胞. 右上
secondary antibody review -- data from 99 publications
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