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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Alpha-synuclein,Snca
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Alpha-synuclein, SNCA ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Alpha-synuclein,Non-A beta component of AD amyloid,Non-A4 component of amyloid precursor,NACP, SNCA,syn,NACP; PARK1; PARK4; PD1; alpha-synuclein; non A-beta component of AD amyloid; synuclein alpha-140; synuclein alpha,non A4 component of amyloid precursor
Search name
Rat Alpha-synuclein ELISA KIT ,Rat Non-A beta component of AD amyloid ELISA KIT ,Rat Non-A4 component of amyloid precursor ELISA KIT ,Rat NACP ELISA KIT ,Rat SNCA ELISA KIT ,Rat syn ELISA KIT ,Rat NACP ELISA KIT ,Rat PARK1 ELISA KIT ,Rat PARK4 ELISA KIT ,Rat PD1 ELISA KIT ,Rat alpha-synuclein ELISA KIT ,Rat non A-beta component of AD amyloid ELISA KIT ,Rat synuclein alpha-140 ELISA KIT ,Rat synuclein alpha ELISA KIT ,Rat non A4 component of amyloid precursor ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Alpha-synuclein concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-synuclein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Alpha-synuclein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Alpha-synuclein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Alpha-synuclein in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验人α-突触核蛋白(a-Synuclein)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人 α- 突触核蛋白 ( a -Synuclein)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 a -Synuclein 单抗包被于酶标板上,标准品和样品中的
。当我们将已知基因作为诱饵,在选定的cDNA文库中筛选与诱饵蛋白相互作用的蛋白,从筛选到的阳性酵母菌株中可以分离得到AD-LIBRARY载体,并从载体中进一步克隆得到随机插入的cDNA片段,并对该片段的编码序列在GENEBANK中进行比较,研究与已知基因在生物学功能上的联系。 另外,也可作为研究已知基因的新功能或多个筛选到的已知基因之间功能相关的主要方法。例如:Engelender等人以神经末端蛋白alpha-synuclein蛋白为诱饵蛋白,利用酵母
。当我们将已知基因作为诱饵,在选定的cDNA文库中筛选与诱饵蛋白相互作用的蛋白�由秆〉降难粜越湍妇�曛锌梢苑掷氲玫�D-LIBRARY载体,并从载体中进一步克隆得到随机插入的cDNA片段,并对该片段的编码序列在GENEBANK中进行比较,研究与已知基因在生物学功能上的联系。另外,也可作为研究已知基因的新功能或多个筛选到的已知基因之间功能相关的主要方法。例如:Engelender等人以神经末端蛋白alpha-synuclein 蛋白为诱饵蛋白,利用酵母双杂交CLONTECH MATCHMARKER
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