- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-17153r
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat 3-ketoacyl-CoA thiolase,mitochondrial,Acaa2
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat 3- ketoacyl- CoA thiolase, mitochondrial, ACAA2 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
ACAA2,mitochondrial 3 oxoacyl CoA thiolase,DSAEC; 3-ketoacyl-CoA thiolase, mitochondrial; acetyl-Coenzyme A acyltransferase 2; beta ketothiolase; beta-ketothiolase; mitochondrial 3-oxoacyl-CoA thiolase; mitochondrial 3-oxoacyl-Coenzyme A thiolase; acetyl-CoA acyltransferase 2
Search name
Rat ACAA2 ELISA KIT ,Rat mitochondrial 3 oxoacyl CoA thiolase ELISA KIT ,Rat DSAEC ELISA KIT ,Rat 3-ketoacyl-CoA thiolase mitochondrial ELISA KIT ,Rat acetyl-Coenzyme A acyltransferase 2 ELISA KIT ,Rat beta ketothiolase ELISA KIT ,Rat beta-ketothiolase ELISA KIT ,Rat mitochondrial 3-oxoacyl-CoA thiolase ELISA KIT ,Rat mitochondrial 3-oxoacyl-Coenzyme A thiolase ELISA KIT ,Rat acetyl-CoA acyltransferase 2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat 3- ketoacyl- CoA thiolase, mitochondrial, ACAA2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to ACAA2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for ACAA2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain ACAA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ACAA2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验脂酰CoA（β-hydroxyacyl CoA）。 （3）再脱氢： β-羟脂酰CoA在β-羟脂酰CoA脱氢酶（L-β-hydroxyacyl CoA dehydrogenase）催化下，脱去β碳上的2个氢原子生成β-酮脂酰CoA，脱下的氢由该酶的辅酶NAD+接受，生成NADH和H+。后者经电子传递链氧化生成水及2.5分子ATP。 （4）硫解： β-酮脂酰CoA在β-酮脂酰CoA硫解酶（β-ketoacyl CoA thiolase）催化下，加一分子CoA-SH，使碳链断裂，产生乙酰CoA
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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