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- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Neudesin,Nenf
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Neudesin, NENF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY!
Alternative name
Rat NENF ELISA KIT ,Rat CIR2 ELISA KIT ,Rat SCIRP10 ELISA KIT ,Rat SPUF ELISA KIT ,Rat SCIRP10-related protein ELISA KIT ,Rat Spinal cord injury related protein 10 ELISA KIT ,Rat cell growth-inhibiting protein 47 ELISA KIT ,Rat cell immortalization-related protein 2 ELISA KIT ,Rat neuron-derived neurotrophic factor ELISA KIT ,Rat secreted protein of unknown function ELISA KIT ,Rat neudesin neurotrophic factor ELISA KIT ,Rat Neudesin ELISA KIT ,Rat SPUF protein ELISA KIT
Intended use
Rat NENF ELISA KIT allows for the in vitro quantitative determination of rat Neudesin concentrations in serum, Plasma,tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat NENF ELISA KIT has been pre-coated with an antibody specific to Neudesin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forNeudesin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Neudesin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Neudesin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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