- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELI-03184r
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Cytochrome P450 2E1,Cyp2e1
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Cytochrome P450 2E1, Cyp2e1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Cytochrome P450 2E1,Cytochrome P450RLM6,Cytochrome P450-J,4-nitrophenol 2-hydroxylase,CYPIIE1, Cyp2e1,cytochrome P450 2E1; CPE1; CYP2E; P450-J; P450C2E; 4-nitrophenol 2-hydroxylase; CYPIIE1; cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1; cytochrome P450-J; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; cytochrome P450 family 2 subfamily E polypeptide 1
Search name
Rat Cytochrome P450 2E1 ELISA KIT ,Rat Cytochrome P450RLM6 ELISA KIT ,Rat Cytochrome P450-J ELISA KIT ,Rat 4-nitrophenol 2-hydroxylase ELISA KIT ,Rat CYPIIE1 ELISA KIT ,Rat Cyp2e1 ELISA KIT ,Rat cytochrome P450 2E1 ELISA KIT ,Rat CPE1 ELISA KIT ,Rat CYP2E ELISA KIT ,Rat P450-J ELISA KIT ,Rat P450C2E ELISA KIT ,Rat 4-nitrophenol 2-hydroxylase ELISA KIT ,Rat CYPIIE1 ELISA KIT ,Rat cytochrome P450 subfamily IIE ethanol-inducible polypeptide 1 ELISA KIT ,Rat cytochrome P450-J ELISA KIT ,Rat flavoprotein-linked monooxygenase ELISA KIT ,Rat microsomal monooxygenase ELISA KIT ,Rat xenobiotic monooxygenase ELISA KIT ,Rat cytochrome P450 family 2 subfamily E polypeptide 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Cytochrome P450 2E1 concentrations in serum, Plasma,, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2E1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Cytochrome P450 2E1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cytochrome P450 2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Cytochrome P450 2E1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Genotyping for Cytochrome P450 Polymorphisms
Protocols for the extraction of DNA from human blood and for genotyping for a number of common cytochrome P450 polymorphisms using either polymerase chain reaction (PCR)-restriction fragment length polymorphism or PCR-single-strand
Cytochrome P450 Reconstitution Systems
Human liver microsomes contain multiple forms of cytochrome P450 (CYP or P450) that catalyze oxidation of a number of xenobiotic and endobiotic chemicals. Individual P450 forms have unique, but overlapping, substrate specificities
Evaluation of Cytochrome P450 Activities in Human Hepatocytes In Vitro
Major hepatic cytochrome P450 activities (CYP1A2, CYP2A6, CYP2B6, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4) can be simultaneously examined in human hepatocytes by incubation with a cocktail of multiple specific probes. Cocktail strategy
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