Rat Thioredoxin, Txn ELISA试剂盒

Rat Thioredoxin, TXN ELISA Kit
96 Tests
Operating instruction

FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!

Synonyms
Thioredoxin(Trx),ATL-derived factor(ADF),Surface-associated sulphydryl protein(SASP), Thioredoxin,TRX; TRDX; TRX1; ADF;
ATL-derived factor; SASP; TXN delta 3; surface-associated sulphydryl protein; thioredoxin delta 3

Search name
Rat Thioredoxin ELISA Kit, Rat Trx ELISA Kit, Rat ATL-derived factor ELISA Kit, Rat ADF ELISA Kit, Rat Surface-associated
sulphydryl protein ELISA Kit, Rat SASP ELISA Kit, Rat TRDX ELISA Kit, Rat Trx1 ELISA Kit, Rat TXN delta 3 ELISA Kit, Rat
thioredoxin delta 3 ELISA Kit

Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat thioredoxin,Trx concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.

Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Trx. Standards or samples are then added to the
appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Trx and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain Trx, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Trx in the samples is then determined by comparing the
O.D. of the samples to the standard curve.

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