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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Heme oxygenase 1,Hmox1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Heme oxygenase 1, Hmox1 ELISA KIT
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Heme oxygenase 1,P32 protein, HMOX1,HO-1,HMOX1D,HSP32,bK286B10,heat shock protein, 32-kD,heme oxygenase 1,heme oxygenase decycling 1
Search name
Rat Heme oxygenase 1 ELISA KIT ,Rat P32 protein ELISA KIT ,Rat HMOX1 ELISA KIT ,Rat HO-1 ELISA KIT ,Rat HMOX1D ELISA KIT ,Rat HSP32 ELISA KIT ,Rat bK286B10 ELISA KIT ,Rat heat shock protein ELISA KIT ,Rat 32-kD ELISA KIT ,Rat heme oxygenase 1 ELISA KIT ,Rat heme oxygenase decycling 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat heme oxygenase 1,HO-1 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to HO-1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for HO-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain HO-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of HO-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验diabetic rat increases infarct size associated with low levels of myocardial HO-1 during ischemia/reperfusion JOURNAL Diabetes 54 (3), 803-810 (2005) PUBMED 15734859 REMARK GeneRIF: This study investigated the role of heme oxygenase (HO
secondary antibody review -- data from 99 publications
western blot test Benzonase activity in PMCA reactions containing biotin-labeled oligo(dT), 17 goat IgG FITC flow cytometry 1:250 Jackson ImmunoResearch Laboratories 17 goat AP ELISA 1:1000 18 HRP western blot Cell
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[求购]请问哪里可以购rat heme oxygenase-1(rHO-1)的全长cDNA? http://www.dxy.cn/bbs/post/view?bid=67&id=5598968&sty=1&tpg=7&age=0 [求助] http://www.dxy.cn/bbs/post/view?bid=67&id=5570022&sty=1&tpg=12&age=0 [求助] http://www.dxy.cn/bbs/post/view?bid=67&id=5542551
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