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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Collagen alpha-1(I) chain,Col1a1
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Collagen alpha-1(I) chain, COL1A1 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
COL1A1,Collagen alpha-1(I) chain,Alpha-1 type I collagen, type I collagen,EDSC; OI1; OI2; OI3; OI4; alpha-1 type I collagen; alpha1(I) procollagen; collagen alpha 1 chain type I; collagen alpha-1(I) chain preproprotein; collagen of skin, tendon and bone alpha-1 chain; pro-alpha-1 collagen type 1; type I proalpha 1; type I procollagen alpha 1 chain; collagen type I alpha 1
Search name
Rat COL1A1 ELISA KIT ,Rat Collagen alpha-1(I) chain ELISA KIT ,Rat Alpha-1 type I collagen ELISA KIT ,Rat type I collagen ELISA KIT ,Rat EDSC ELISA KIT ,Rat OI1 ELISA KIT ,Rat OI2 ELISA KIT ,Rat OI3 ELISA KIT ,Rat OI4 ELISA KIT ,Rat alpha-1 type I collagen ELISA KIT ,Rat alpha1(I) procollagen ELISA KIT ,Rat collagen alpha 1 chain type I ELISA KIT ,Rat collagen alpha-1(I) chain preproprotein ELISA KIT ,Rat collagen of skin ELISA KIT ,Rat tendon and bone alpha-1 chain ELISA KIT ,Rat pro-alpha-1 collagen type 1 ELISA KIT ,Rat type I proalpha 1 ELISA KIT ,Rat type I procollagen alpha 1 chain ELISA KIT ,Rat collagen type I alpha 1 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Collagen alpha-1(I) chain concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen alpha-1(I) chain. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Collagen alpha-1(I) chain and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Collagen alpha-1(I) chain, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Collagen alpha-1(I) chain in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
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