相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Interleukin-1 beta,Il1b
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Interleukin- 1 beta, IL- 1b ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Interleukin-1 beta(IL-1 beta),;Catabolin,; interleukin-1 beta; IL-1beta; IL1beta; IL-1 beta; interleukin 1, beta
Search name
Rat Interleukin-1 beta ELISA Kit, Rat IL-1b ELISA Kit, Rat Interleukin-1b ELISA Kit, Rat IL-1beta ELISA Kit, Rat
Catabolin ELISA KIT,Rat IL1F2 ELISA KIT, Interleukin-1 beta ELISA Kit, IL-1b ELISA Kit, Interleukin-1b ELISA Kit, IL-1beta
ELISA Kit, Catabolin ELISA KIT, IL1F2 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat interleukin-1 beta,IL-1 beta concentrations in serum,
Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-1 beta. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for IL-1 beta and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain IL-1 beta, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of IL-1 beta in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Western Blot 现在仍然是检测和分离蛋白最常用的一种实验方法,但是要想得到较好的结果并不容易,只有找到合适的实验条件并进行优化,才能以更少的时间得到更多的结果,达到事半功倍的效果!下面是 R&D Systems 品牌为您推荐的免疫印迹优化步骤和实验涉及参考数据:一抗浓度对实验结果的影响抗体:Human/Mouse/Rat RSK1 Antibody (Catalog # AF992)样本:人 HeLa 细胞 (Fig.1)A:5% 脱脂奶粉封闭,一抗浓度:1.0 mg/mL,溶于
AHPGWFICTS CNCNEPVGVT DKFENRKHIEFSFQPVCKAE MSPSEVSD2, FeaturesDL-IL1z-Hu人白介素 1ζ(IL1Z) 酶联免疫吸附检测试剂盒预期应用本试剂盒运用双抗体夹心 ELISA 法定量测定人血清、血浆、组织匀浆、细胞裂解液、细胞培养上清液和其他生物液体中 IL1Z 含量。标本的采集与保存1. 血清:将收集于血清分离管的全血标本在室温放置 2 小时或 4℃ 过夜,然后 1000×g 离心 20 分钟,取上清即可,或将上清置于-20℃ 或-80
【申请上传】Current Protocols in Immunology
Immunocompromised Animals Unit 1.3 Handling and Restraint Unit 1.4 Anesthesia Unit 1.5 Animal Identification Section II Use of Mouse, Rat, Hamster, and Rabbit Unit 1.6 Parenteral Injections Unit 1.7 Blood Collection Unit 1.8 Euthanasia Section III Selected
技术资料暂无技术资料 索取技术资料







