Rat CXC- chemokine receptor 2,CXCR2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CXCR2,CXC-R2; CD182; CDw128b; CMKAR2; IL8R2; IL8RA; IL8RB; C-X-C chemokine receptor type 2; CXCR-2; CXCR2 gene for IL8 receptor type B; GRO/MGSA receptor; IL-8 receptor type 2; IL-8R B; chemokine (CXC) receptor 2; high affinity interleukin-8 receptor B; interleukin 8 receptor B; interleukin 8 receptor type 2; interleukin 8 receptor beta; interleukin-8 receptor type B; chemokine (C-X-C motif) receptor 2 C-X-C chemokine receptor type 2,CXC-R2;CXCR-2,High affinity interleukin-8 receptor B,IL-8R B,
Search name
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Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat C-X-C chemokine receptor type 2, CXC-R2 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to CXC-R2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CXC-R2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain CXC-R2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of CXC-R2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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