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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat c-fos
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat c- fos ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
c-Fos,proto-oncogene c-Fos; cellular oncogene c-fos; AP-1; p55; FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS); G0/G1 switch regulatory protein 7; activator protein 1; FBJ murine osteosarcoma viral oncogene homolog,Proto-oncogene c-Fos,Cellular oncogene fos,G0/G1 switch regulatory protein 7,
Search name
Rat c-Fos ELISA KIT ,Rat CFOS ELISA KIT ,Rat proto-oncogene c-Fos ELISA KIT ,Rat cellular oncogene c-fos ELISA KIT ,Rat AP-1 ELISA KIT ,Rat p55 ELISA KIT ,Rat FBJ murine osteosarcoma viral (v-fos) oncogene homolog ELISA KIT ,Rat oncogene FOS ELISA KIT ,Rat G0/G1 switch regulatory protein 7 ELISA KIT ,Rat activator protein 1 ELISA KIT ,Rat FBJ murine osteosarcoma viral oncogene homolog ELISA KIT ,Rat Proto-oncogene c-Fos ELISA KIT ,Rat Cellular oncogene fos G0/G1 switch regulatory protein 7 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Proto-oncogene c-Fos concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Proto-oncogene c-Fos. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Proto-oncogene c-Fos and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Proto-oncogene c-Fos, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Proto-oncogene c-Fos in the samples is then determined by comparing the O.D. of the samples to the standard curve.
FOS Related Products
> FOS ELISA KITS
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Chicken Proto- oncogene c- Fos, FOS ELISA KIT
Human c- fos ELISA Kit
Human Proto- oncogene c- Fos, FOS ELISA KIT
Mouse Proto- oncogene c- Fos, Fos ELISA KIT
Porcine Proto- oncogene c- Fos, FOS ELISA KIT
Rat c- fos ELISA Kit
Rat Proto- oncogene c- Fos, Fos ELISA KIT
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人C-Fos ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 C-Fos 单抗包被于酶标板上,标准品和样品中的 C-Fos与单抗结合,加入生物素化的抗人 C-Fos ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 C-Fos ELISA 试剂盒 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 C-Fos 单抗包被于酶标板上,标准品和样品中的 C-Fos 与单抗结合,加入生物素化的抗大鼠 C-Fos ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝
1986 年 Maki 等从鸡的肉瘤病毒 ASV17 中分离了一个新的癌基因,叫做 V-Jum, 编码 296 个氨基酸的多肽,表达产物为 p65Kda ,是一种融合蛋白。与小鼠的 C-J um 有 79% 的同源性,人类的 c-jum 和小鼠的 c-jum 有 90% 的同源性。 人类的 c-jum 基因定位于 14q21-31 ,成熟的 mRNA 长 2.2Kb ,编码含 380 个氨基酸的 p55c-fos
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