- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0899r
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Osteopontin,OPN
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Osteopontin, OPN ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Osteopontin,Uropontin,Urinary stone protein,Secreted phosphoprotein 1(SPP-1),Bone sialoprotein 1,Nephropontin, osteopontin,secreted
phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); osteopontin/immunoglobulin alpha 1 heavy chain
constant region fusion protein; BNSP; BSPI; ETA-1; OPN; SPP1/CALPHA1 fusion; early T-lymphocyte activation 1; nephropontin; urinary
stone protein; uropontin; secreted phosphoprotein 1
SearCh name
Rat Osteopontin ELISA Kit, Rat OPN ELISA Kit, Rat Uropontin ELISA Kit, Rat Urinary stone protein ELISA Kit,
Rat SeCreted phosphoprotein 1 ELISA Kit, Rat SPP-1 ELISA Kit, Rat Bone sialoprotein 1 ELISA Kit, Rat
Nephropontin ELISA Kit, Rat bone sialoprotein I ELISA Kit, Rat early T-lymphoCyte aCtivation 1 ELISA Kit, Rat
immunoglobulin alpha 1 heavy Chain Constant region fusion protein ELISA Kit, Rat BNSP ELISA Kit, Rat BSPI ELISA Kit,
Rat ETA-1 ELISA Kit, Rat SPP1 ELISA Kit, Rat CALPHA1 fusion ELISA Kit, Rat early T-lymphoCyte aCtivation 1
ELISA Kit, Rat nephropontin; urinary stone protein ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat Osteopontin concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Osteopontin. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Osteopontin and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Osteopontin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Osteopontin in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验大鼠 骨桥素(OPN ) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆及相关液体样本 中 骨桥素(OPN ) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中 大鼠 骨桥素(OPN ) 水 平。用纯化的 大鼠 骨桥素(OPN ) 抗体 包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 骨桥素(OPN ) ，再与 HRP 标记
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人骨桥素 ( OPN )ELISA 试剂盒 ( 用于母乳、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 OPN 单抗包被于酶标板上，标准品和样品中的 OPN与单抗结合，加入生物素化的抗人 OPN ，形成免疫复合物连接
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 豚鼠骨桥素 ( OPN )ELISA 试剂盒 ( 用于母乳、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗豚鼠 OPN 单抗包被于酶标板上，标准品和样品中的 OPN与单抗结合，加入生物素化的抗豚鼠 OPN ，形成
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