- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0753r
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Toll-like receptor 4,TLR4
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Toll-like receptor 4, TLR4 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Toll-like receptor 4, TLR4,LPS,hToll,CD284,ARMD10; TLR-4; TOLL; homolog of Drosophila toll;
Search name
Human Toll-like receptor 4 ELISA KIT ,Human TLR4 ELISA KIT ,Human LPS ELISA KIT ,Human hToll ELISA KIT ,Human CD284 ELISA KIT ,Human ARMD10 ELISA KIT ,Human TLR-4 ELISA KIT ,Human TOLL ELISA KIT ,Human homolog of Drosophila toll ELISA KIT, Rat Toll4 ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat toll-like receptor 4,Toll4 concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Toll4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Toll4 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Toll4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Toll4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Analysis of the Functional Role of Toll-Like Receptor-4 Tyrosine Phosphorylation
role of TLR4 tyrosine phosphorylation in receptor signaling, including transient transfections, site-directed mutagenesis, immunoprecipitation and immunoblot analyses, and analyses of transcription factor activation in reporter assays.
Practical Techniques for Detection of Toll-Like Receptor-4 in the Human Intestine
to be able to respond to pathogenic organisms while at the same time maintain tolerance to normal commensal flora. Toll-like receptors (TLRs) play an important role in this delicate balance. TLRs are transmembrane noncatalytic receptor proteins that induce
【信息】The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling
Review Nature Reviews Immunology 7, 353-364 (May 2007) | doi:10.1038/nri2079 The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling Luke A. J. O'Neill1 & Andrew G. Bowie1 About the authors Summary TLR (Toll-like
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