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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat cyclooxygenase-2,COX-2
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat cyclooxygenase- 2, COX- 2 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Prostaglandin G/H synthase 2,;
PHS II,PES-2,Macrophage activation-associated marker protein P71/73,Gripghs,TIS10 protein,Prostaglandin-endoperoxide synthase
2,Prostaglandin H2 synthase 2(PGH synthase 2,PGHS-2),Cyclooxygenase-2(COX-2),Glucocorticoid-regulated inflammatory
cyclooxygenase,
Search name
Rat Prostaglandin G/H synthase 2 ELISA Kit, Rat cyclooxygenase- 2 ELISA Kit
Rat COX- 2 ELISA Kit, Rat Prostaglandin-endoperoxide synthase 2 ELISA Kit, Rat Prostaglandin H2 synthase 2ELISA Kit, Rat
PGH synthase 2 ELISA Kit, Rat PGHS-2 ELISA Kit, Rat PHS II ELISA Kit, Rat PES-2 ELISA Kit, Rat Macrophage
activation-associated marker protein P71/73 ELISA Kit, Rat TIS10 protein ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat prostaglandin g/h synthase 2,Prostaglandin G/H synthase 2
concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Prostaglandin G/H synthase 2. Standards or
samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for
Prostaglandin G/H synthase 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated.
Then a TMB substrate solution is added to each well. Only those wells that contain Prostaglandin G/H synthase 2, biotin-conjugated
antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a
sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of
Prostaglandin G/H synthase 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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