- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0632r
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Pepsin,PP
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Pepsin, PP ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Pepsin A, PGA5,pepsin A-5; pepsin A; PGA3; PGA4; pepsinogen A5; pepsinogen-5; pepsinogen 5, group I (pepsinogen A)
Search name
Rat Pepsin A ELISA Kit, Rat Pepsin ELISA Kit,Rat PGA5 ELISA KIT,Rat pepsin A-5 ELISA KIT,Rat PGA3 ELISA
KIT, Rat pepsinogen A5 ELISA KIT, Rat pepsinogen-5 ELISA KIT, Rat pepsinogen 5 ELISA KIT, Pepsin A ELISA Kit,
Pepsin ELISA Kit, PGA5 ELISA KIT,pepsin A-5 ELISA KIT, PGA3 ELISA KIT,pepsinogen A5 ELISA KIT,pepsinogen-5 ELISA KIT,
pepsinogen 5 ELISA KIT,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat pepsin a,Pepsin A concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pepsin A. Standards or samples are then added
to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Pepsin A and Avidin
conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to
each well. Only those wells that contain Pepsin A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color.
The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Pepsin A in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
-PCR 5. forward primer： 5’-TCACTCAAGATTGTCAGCAA-3’ reverse primer: 5'-AGATCCACGACGGACACATT-3' 6. 产物长度（bp）: 308bp 7. 引物浓度：0.25umol/L 8. 退火温度：58℃ 1. 物种：rat 2. 基因名称：内参β-actin 3. 模板：cDNA 4. PCR类型及目的：RT-PCR 5. sense 5'-ATG CCA TCC TGC GTC TGG ACC TGG C
大鼠胃蛋白酶(Pepsin) 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，血浆，细胞上清及相关液体样本中胃蛋白酶(Pepsin) 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中大鼠胃蛋白酶 (Pepsin) 水平。用纯化的大鼠胃蛋白酶 (Pepsin) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入胃蛋白酶 (Pepsin) ，再与 HRP 标记
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