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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Soluble protein 100B
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Soluble protein 100B, S100B ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Protein S100-B,S100 calcium-binding protein B,S-100 protein beta chain,S-100 protein subunit beta, S100B,protein S100-B; NEF; S100;
S100-B; S100beta; S-100 calcium-binding protein, beta chain; S-100 protein subunit beta; S100 calcium-binding protein, beta (neural);
S100 calcium binding protein B
Search name
Rat Protein S100-B ELISA Kit, Rat Soluble protein-100B ELISA Kit, Rat S-100B ELISA Kit, Rat S100
calcium-binding protein B ELISA Kit, Rat S-100 protein beta chain ELISA Kit, Rat S-100 protein subunit beta ELISA Kit,
Rat S100B ELISA Kit, Rat protein S100-B ELISA Kit, Rat NEF ELISA Kit, Rat S100 ELISA Kit, Rat S100-B ELISA
Kit, Rat S100beta ELISA Kit, Rat S-100 calcium-binding protein beta chain ELISA Kit, Rat S-100 protein subunit beta
ELISA Kit, Rat S100 calcium-binding protein, beta (neural) ELISA Kit, Rat S100 calcium binding protein B ELISA Kit
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Rat Protein S100-B concentrations in serum, Plasma, tissue
homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protein S100-B. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Protein S100-B and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Protein S100-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Protein S100-B in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
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文献和实验at 4°C for 15 minutes. 9. Remove and aliquot supernatant. We recommend making several 50 μl aliquots. 10. Before running ELISA, dilute protein 1:100 and run a Bradford total protein assay or Quant-iT™ Protein Quantitation Kit assay (Cat. no. Q
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cathy12303 有没有哪位前辈用过cAMP的ELISA试剂盒? dianaofyezi 我 有问题可以站内消息或者QQ52187644联系。 白大褂2009 你好,我是新手,你用过的ELISA试剂在哪买的,哪个公司的比较好啊?还有,测定大鼠脑组织里的cAMP,放免法和ELISA法各有什么优缺点啊?谢谢回复!! songdianwei 我在 R&D公司买的,测
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