- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- ELA-E0263r
- 2026年01月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat advanced glycation end products,AGEs
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat advanced glycation end products, AGEs ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Rat AGES ELISA KIT ,Rat AGE ELISA KIT ,Rat Advanced glycation end-products ELISA KIT ,Rat Advanced glycation end product ELISA KIT
Intended use
Rat AGES ELISA KIT allows for the in vitro quantitative determination of rat Advanced glycation end-product concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Rat AGES ELISA KIT is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Advanced glycation end-product, During the reaction, Advanced glycation end-product in the sample or standard competes with a fixed amount of biotin-labeled Advanced glycation end-product for sites on a pre-coated Monoclonal antibody specific to Advanced glycation end-product. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Advanced glycation end-product in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Range 0.78-50ng/ml
Rat AGES ELISA KIT Manual download
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文献和实验Advanced glycation endproducts (AGEs) are stable end-stage adducts formed by the nonenzymatic reaction of saccharide derivatives with proteins, nucleotides, and phospholipids. They are formed in physiological systems by glycation reactions
Autofluorescent Ceroid/Lipofuscin
.g., in the kidney, thymus, pancreas, testis, prostate, seminal vesicles, uterus, and adrenal gland of man and animal (1 –4 ). Advanced glycation end-products (AGEs) are mainly found extracellularly in association with long-lived proteins, e.g
. The more important protein modifications are referred to as advanced glycation end products (AGEs) and advanced lipoxidation end products (ALEs). Protein modification can result in both non-cross-link and cross-link AGEs and ALEs, the latter arising
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