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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Granulocyte macrophage colony stimulating factor,GM-CSF EL
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Granulocyte macrophage colony stimulating factor, GM- CSF ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Granulocyte-macrophage colony-stimulating factor,GM-CSF,Colony-stimulating factor,CSF, CSF2,GM-CSF,GMCSF; CSF; granulocyte-macrophage colony-stimulating factor; molgramostin; sargramostim; colony stimulating factor 2,granulocyte-macrophage
Search name
Rat Granulocyte-macrophage colony-stimulating factor ELISA KIT ,Rat GM-CSF ELISA KIT ,Rat Colony-stimulating factor ELISA KIT ,Rat CSF ELISA KIT ,Rat CSF2 ELISA KIT ,Rat GM-CSF ELISA KIT ,Rat GMCSF ELISA KIT ,Rat CSF ELISA KIT ,Rat granulocyte-macrophage colony-stimulating factor ELISA KIT ,Rat molgramostin ELISA KIT ,Rat sargramostim ELISA KIT ,Rat colony stimulating factor 2 ELISA KIT ,Rat granulocyte-macrophage ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of rat granulocyte-macrophage colony-stimulating factor,GM-CSF concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM-CSF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GM-CSF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GM-CSF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GM-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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文献和实验Acute myeloid leukemia (AML) has an annual incidence of 2.4 cases per 100,000 persons and is the most common form of acute leukemia in adults (1 , 2 ). AML is a model for drug-resistant human cancers, and current therapies for AML possess
The hematopoietic growth factor granulocyte colony-stimulating factor (G-CSF) has been in the clinic since 1986, when the clinical development of bacterially synthesized recombinant filgrastim began (1 ). A glycosylated form of G-CSF
碱性粒细胞的增殖,阻止肥大细胞发生程序性细胞死亡。 (二)GM-CSF 1977年Burgess等从小鼠肺条件培养液中发现一种能刺激粒细胞和巨噬细胞形成集落的因子,命名为粒细胞-巨噬细胞集落刺激因子(granulocyte-macrophage colony stimulating factor,GM-CSF)。1984年和1985年小鼠和人GM-CSF的cDNA分别克隆成功。 1.GM-CSF的产生 T细胞、B细胞、巨噬细胞、肥大细胞、内皮细胞、成纤维细胞等均可产生GM-CSF
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