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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
78-5000pg/ml
- 检测方法:
夹心法
- 应用:
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种:
大鼠
- 标记物:
Rat Homocysteine,Hcy
- 样本:
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
46.9pg/ml
- 规格:
96tests
Rat Homocysteine, Hcy ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Homocysteine,Homocysteic acid,HCY, Homocysteine acid
Search name
Rat Homocysteine ELISA Kit, Rat Homocysteic acid ELISA Kit, Rat HCY ELISA Kit, Rat Homocysteine acid ELISA Kit
Intended use
Rat Homocysteine ELISA Kit allows for the in vitro quantitative determination of homocysteine,Homocysteine concentrations in serum, Plasma,
tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in Rat Homocysteine ELISA Kit has been pre-coated with an antibody specific to Homocysteine. Standards or samples are then
added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Homocysteine and
Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is
added to each well. Only those wells that contain Homocysteine, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a
change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is
measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Homocysteine in the samples is then
determined by comparing the O.D. of the samples to the standard curve.
Rat Homocysteine ELISA Kit PDF manual download
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文献和实验8 Determination of Plasma Homocysteine
A number of epidemiological and clinical studies have linked elevated plasma homocysteine (Hcy) to atherosclerotic vascular disease affecting coronary, carotid, and peripheral vessels. Plasma Hcy can be considered a marker of methionine
S-Adenosyl-L-Homocysteine Hydrolase
The enzymatic activity that catalyzes the reversible hydrolysis of S-adenosyl-L -homocysteine (AdoHcy) to L -homocysteine (Hey) and adenosine (Ado), or, in reverse, the formation of AdoHcy from Hcy and Ado, is known as AdoHcy hydrolase (AHH
Total Plasma Homocysteine Analysis by HPLC with SBD-F Precolumn Derivatization
why homocysteine (Hcy) measurement is included in he list of tests for investigating the causes of atherosclerosis and thrombosis.
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