- ￥2980
- LSM Bio
- 78-5000pg/ml
- 46.9pg/ml
- 进口
- CELI-66018r
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
78-5000pg/ml
- 检测方法：
夹心法
- 应用：
检测大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 适应物种：
大鼠
- 标记物：
Rat Diacylglycerol,DAG
- 样本：
大鼠血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
46.9pg/ml
- 规格：
96tests
Rat Diacylglycerol, DAG ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Diacylglycerol, Diglyceride, DAG
Search name
Rat Diacylglycerol, DAG ELISA Kit, Rat DAG ELISA Kit, Rat Diglyceride ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of
diacylglycerol,Diacylglycerol concentrations in serum, plasma,
tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been
pre-coated with an antibody specific to Diacylglycerol, During the reaction, Diacylglycerol in the sample or standard competes with a fixed
amount of biotin-labeled Diacylglycerol for sites on a pre-coated Monoclonal antibody specific to Diacylglycerol. Excess conjugate and
unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each
microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The
concentration of Diacylglycerol in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF manual download
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文献和实验Measurement of Diacylglycerol: Origins of Receptor- Stimulated Diacylglycerol Accumulation
Diacylglycerol (DAG) found within cells is derived from two broad sources. First, in the de novo biosynthetic pathway, glycerol 3-phosphate is converted by two acylation steps to phosphatidic acid (PA), which is converted to DAG by phosphatidate
Measurement of Cellular Diacylglycerol Content
of the resultant second messengers, inositol 1,4,5-trisphosphate and diacylglycerol (DAG), became essential. The additional discovery of an effector enzyme, protein kinase C, for transducing the changes in DAG levels (reviewed in ref. 2 ) underlined the importance
Quantification of Diacylglycerol by Mass Spectrometry
Diacylglycerol (DAG) is an important intermediate of lipid metabolism and a component of phospholipase C signal transduction. Quantification of DAG in plant membranes represents a challenging task because of its low abundance. DAG
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