- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELA-E0553b
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine matrix metalloproteinase 9/ Gelatinase B,MMP-9 ELISA Ki
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine matrix metalloproteinase 9/ Gelatinase B, MMP- 9 ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
Matrix metalloproteinase-9(MMP-9),Gelatinase B(GELB),92 kDa gelatinase,92 kDa type IV collagenase, MMP-9,matrix metallopeptidase
9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase); matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type
IV collagenase); CLG4B; GELB; MANDP2; 92 kDa gelatinase; 92 kDa type IV collagenase; macrophage gelatinase; matrix
metalloproteinase-9; type V collagenase
Search name
Bovine matrix metalloproteinase 9 ELISA Kit, Bovine MMP- 9 ELISA Kit, Bovine Gelatinase B ELISA Kit, Bovine GELB ELISA Kit,
Bovine 92 kDa gelatinase ELISA Kit, Bovine 92 kDa type IV collagenas ELISA Kit, Bovine CLG4B ELISA Kit, Bovine MANDP2
ELISA Kit, Bovine macrophage gelatinase ELISA Kit, Bovine type V collagenase ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine matrix metalloproteinase-9,MMP-9 concentrations in
serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-9. Standards or samples are then added to
the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP-9 and Avidin conjugated to
Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well.
Only those wells that contain MMP-9, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The
enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of MMP-9 in the samples is then determined by comparing
the O.D. of the samples to the standard curve.
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