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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Gap junction alpha-4 protein,GJA4
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Gap junction alpha- 4 protein, GJA4 ELISA KIT
Product Name:Bovine Gap junction alpha- 4 protein, GJA4 ELISA KIT
Packing:96T
Catalog No.:ELI-08898b
Gene Name:Bovine Gap junction alpha-4 protein, GJA4
Detect Range:31.2-2000pg/ml
Sensitivity:18.75pg/ml
Target Protein Name:Bovine Gap junction alpha-4 protein, GJA4
Alternative Name:GJA4,Bovine Gap junction alpha-4 protein, GJA4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Gap junction alpha- 4 protein, GJA4 ELISA KIT allows for the in vitro quantitative determination of Bovine Gap junction alpha-4 protein, GJA4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Gap junction alpha- 4 protein, GJA4 ELISA KIT has been pre-coated with an Bovine Gap junction alpha-4 protein, GJA4 antibody specific to Bovine Gap junction alpha-4 protein, GJA4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Gap junction alpha-4 protein, GJA4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Gap junction alpha-4 protein, GJA4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Gap junction alpha-4 protein, GJA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
9600 thermal cycler with thin-walled 0.2 ml tubes, and AmpliTaq polymerase from Perkin-Elmer. 6.) Materials for preparative agarose gel electrophoresis. We use the Quiaquick gel extraction kit (Qiagen) for all band excision steps, and the Quiaquick
Functional Stem Cell Integration Assessed by Organotypic Slice Cultures
neural stem cells (NSCs) filled with the gap junction‐permeable dye calcein (green) and the gap junction‐impermeable dye DiI (red) following engraftment to an organotypic striatal tissue culture. Cell nuclei are displayed by DAPI nuclear counterstain
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
Daly, D.S., Anderson, K.K., White, A.M., Gonzalez, R.M., Varnum, S.M., and Zangar, R.C. 2008. Predicting protein concentrations with ELISA microarray assays, monotonic splines and Monte Carlo simulation. Stat. Appl. Genet. Mol. Biol. 7: Article21
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