- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-06855b
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine Telomerase reverse transcriptase,TERT
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine Telomerase reverse transcriptase, TERT ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
TERT,CMM9; DKCA2; DKCB4; EST2; PFBMFT1; TCS1; TP2; TRT; hEST2; hTRT; telomerase catalytic subunit; telomerase-associated protein 2; telomerase reverse transcriptase,Telomerase reverse transcriptase,Telomerase-associated protein 2,TP2,HEST2,Telomerase catalytic subunit,
Search name
Bovine TERT ELISA KIT ,Bovine CMM9 ELISA KIT ,Bovine DKCA2 ELISA KIT ,Bovine DKCB4 ELISA KIT ,Bovine EST2 ELISA KIT ,Bovine PFBMFT1 ELISA KIT ,Bovine TCS1 ELISA KIT ,Bovine TP2 ELISA KIT ,Bovine TRT ELISA KIT ,Bovine hEST2 ELISA KIT ,Bovine hTRT ELISA KIT ,Bovine telomerase catalytic subunit ELISA KIT ,Bovine telomerase-associated protein 2 ELISA KIT ,Bovine telomerase reverse transcriptase ELISA KIT ,Bovine Telomerase reverse transcriptase ELISA KIT ,Bovine Telomerase-associated protein 2 ELISA KIT ,Bovine TP2 ELISA KIT ,Bovine HEST2 ELISA KIT ,Bovine Telomerase catalytic subunit ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of bovine Telomerase reverse transcriptase concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to Telomerase reverse transcriptase. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Telomerase reverse transcriptase and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Telomerase reverse transcriptase, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Telomerase reverse transcriptase in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF
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文献和实验Methods of Telomerase Inhibition
as a possible anticancer approach. Many of the methods applied to telomerase inhibition focus on either of the two major components of the ribonucleoprotein holoenzyme, that is, the telomerase reverse transcriptase (TERT) catalytic subunit or the telomerase RNA
Analysis of Yeast Telomerase by Primer Extension Assays
, the consequence of telomerase loss was first characterized in the budding yeast Saccharomyces cerevisiae (Lundblad and Szostak , Cell 57:633–643, 1989). The catalytic component of telomerase (telomerase reverse transcriptase; TERT) was likewise initially cloned
Assessing the Involvement of Telomerase in Stem Cell Biology
) and a protein called TERT that possesses reverse transcriptase activity. Several telomere-associated proteins have been identified including TRF1 (telomere repeat-binding factor 1) that may inhibit telomerase activity and promote telomere shortening, and TRF
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