- ￥3480
- LSM Bio
- 0.78-50ng/ml
- 0.469ng/ml
- 进口
- ELI-05469b
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
100
- 供应商：
LSM BIO
- 检测范围：
0.78-50ng/ml
- 检测方法：
夹心法
- 应用：
利用ELISA方法体外定量检测牛血清，血浆等样本中目标蛋白的浓度
- 适应物种：
牛
- 标记物：
Bovine 5'-AMP-activated protein kinase subunit beta-1,AMPK ELIS
- 样本：
牛的血浆，血清，细胞上清等
- 灵敏度：
0.469ng/ml
- 规格：
96tests
Bovine 5'-AMP-activated protein kinase subunit beta-1, AMPK ELISA Kit
96 Tests
Operating instruction
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE
BEGINNING!
Synonyms
5'-AMP-activated protein kinase subunit beta-1,AMPK subunit beta-1,AMPKb,,AMPK,HAMPKb,5'-AMP-activated protein kinase beta-1
subunit,5'-AMP-activated protein kinase subunit beta-1,AMP-activated protein kinase beta subunit,AMPK beta -1 chain,AMPK beta
1,AMPK subunit beta-1,AMPKb,protein kinase, AMP-activated, noncatalytic, beta-1,protein kinase, AMP-activated, beta 1 non-catalytic
subunit
Search name
Bovine 5'-AMP-activated protein kinase subunit beta-1 ELISA Kit,Bovine PRKAB ELISA Kit, 5'-AMP-activated protein kinase
subunit beta-1 ELISA Kit,PRKAB ELISA Kit,ELISA Kit,5'-AMP-activated protein kinase subunit beta-1 ELISA Kit,AMPK subunit
beta-1 ELISA Kit,AMPKb ELISA Kit, ELISA Kit,AMPK ELISA Kit,HAMPKb ELISA Kit,5'-AMP-activated protein kinase beta-1
subunit ELISA Kit,5'-AMP-activated protein kinase subunit beta-1 ELISA Kit,AMP-activated protein kinase beta subunit ELISA
Kit,AMPK beta -1 chain ELISA Kit,AMPK beta 1 ELISA Kit,AMPK subunit beta-1 ELISA Kit,AMPKb ELISA Kit,protein kinase ELISA
Kit, AMP-activated ELISA Kit, noncatalytic ELISA Kit, beta-1 ELISA Kit,protein kinase ELISA Kit, AMP-activated ELISA Kit, beta 1
non-catalytic subunit ELISA Kit,
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Bovine 5'-amp-activated protein kinase subunit beta-1,AMPK
subunit beta-1 concentrations in serum, Plasma, Urine, tissue homogenates and Cell culture supernates and Other biological fluids.
Test principle
The microtiter plate provided in this kit has been pre-coated with an antibody specific to AMPK subunit beta-1. Standards or samples are
then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for AMPK subunit
beta-1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate
solution is added to each well. Only those wells that contain AMPK subunit beta-1, biotin-conjugated antibody and enzyme-conjugated
Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of AMPK subunit beta-1 in the samples
is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验Analysis of the Role of the AMP-Activated Protein Kinase in the Response to Cellular Stress
The AMP-activated protein kinase (AMPK) is the central component of a protein kinase cascade that is activated by cellular stresses causing ATP depletion and has been referred to as a “fuel gauge” or “metabolic sensor” of the eukaryotic cell
Nature 重磅：林圣彩院士团队七年攻坚！首次破解「神药」二甲双胍作用靶点之谜
截至目前，二甲双胍发挥作用的多种机制被提出。比如说，二甲双胍可以抑制肝细胞线粒体电子传递链的复合物 I，从而降低线粒体氧耗，并抑制三磷酸腺苷（ATP）的生成，使 AMP/ATP 比值升高。增加的 AMP 还可以抑制果糖-1,6-二磷酸酶-1 和腺苷酸环化酶，从而阻止糖异生；二甲双胍也被认为可以改变细胞氧化还原状态，从而增加 NAD+/NADH 比值，抑制糖异生底物的利用。 在二甲双胍的各种潜在效应物中，腺苷酸活化蛋白激酶（AMP-activated protein kinase , AMPK
三句话读懂一篇 CNS：咖啡竟可降低心律失常风险；转入肥胖基
AMPK（AMP-activated protein kinase）是细胞内最重要的能量感受器和调节器，其功能失常会导致人体罹患肥胖、糖尿病等疾病。2021 年 7 月 23 日，美国温安洛研究所（Van Andel Institute）KarstenMelcher 课题组联合中国科学院上海药物研究所徐华强等多个课题组在 Science 杂志发表论文 Structure of an AMPK complex in its inactive, ATP-bound state。利用 Cryo-EM 技术
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