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- 详细信息
- 文献和实验
- 技术资料
- 库存:
100
- 供应商:
LSM BIO
- 检测范围:
0.78-50ng/ml
- 检测方法:
夹心法
- 应用:
利用ELISA方法体外定量检测牛血清,血浆等样本中目标蛋白的浓度
- 适应物种:
牛
- 标记物:
Bovine Glucose-6-phosphate isomerase,GPI
- 样本:
牛的血浆,血清,细胞上清等
- 灵敏度:
0.469ng/ml
- 规格:
96tests
Bovine Glucose- 6- phosphate isomerase, GPI ELISA KIT
Product Name:Bovine Glucose- 6- phosphate isomerase, GPI ELISA KIT
Packing:96T
Catalog No.:ELI-02514b
Gene Name:Bovine Glucose-6-phosphate isomerase, GPI
Detect Range:0.781-50ng/ml
Sensitivity:0.469ng/ml
Target Protein Name:Bovine Glucose-6-phosphate isomerase, GPI
Alternative Name:GPI,Bovine Glucose-6-phosphate isomerase, GPI
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Bovine Glucose- 6- phosphate isomerase, GPI ELISA KIT allows for the in vitro quantitative determination of Bovine Glucose-6-phosphate isomerase, GPI concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Bovine Glucose- 6- phosphate isomerase, GPI ELISA KIT has been pre-coated with an Bovine Glucose-6-phosphate isomerase, GPI antibody specific to Bovine Glucose-6-phosphate isomerase, GPI .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Bovine Glucose-6-phosphate isomerase, GPI and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Bovine Glucose-6-phosphate isomerase, GPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Bovine Glucose-6-phosphate isomerase, GPI in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验The K/BxN Mouse Model of Inflammatory Arthritis: Theory and Practice
autoantibodies to glucose-6-phosphate isomerase (GPI). This model has been useful for the investigation of the development of autoimmunity (K/B�N transgenic mice) and particularly of the mechanisms by which anti-GPI autoantibodies induce joint-specific
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(100 units/ml and 100 μg/ml respectively) and 1.98 g of glucose to 250 ml 1x sterile phosphate buffered saline (PBS) and bring solution volume to 500 ml with additional sterile 1x PBS. 2) Prepare stop media by adding 25 ml FBS and 5 ml
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